国产成人综合久久久久久,亚洲国产成人久久综合碰,精品露脸国产偷人在视频,99久久精品免费看国产,国产免费一区二区三区在线观看,好吊妞国产欧美日韩免费观看,国产精品免费看久久久无码

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pOSEX5C
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pOSEX5C
pOSEX5C
規(guī)格:
貨期:
編號:B226527
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pOSEX5C
商品貨號 B226527
Designations pOSEX5C
Depositors E Bremer
Biosafety Level 1
Vector Information
Size (kb): 4.4450001716613770
Vector: pOSEX5C (plasmid)
Promoters: Promoter for expression proU
Construction: pOSEX3
Marker(s):ampR
Construct size (kb): 4.445000171661377
Features: initiation codon: ATG
marker(s): ampR
promoter for expression: proU
replicon: pMB1
replicon: rop (copy number control)
MCS: NcoI...HindIII
transcription terminator: rrnB T1
transcription terminator: rrnB T2
coding sequence: proV 5' sequence
Applications
expression vector
vector permitting construction of fusion proteins
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HindIII--4.4; PvuII--4.4.
Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride.
Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter.
Absence of a stop codon between the proV' sequence and the ATG start codon allows production of a fusion protein between ProV and the cloned insert. Insertion of a stop codon at this junction may reduce the yield of recombinant protein.
This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor.
Vector constructed from pOSEX3 (ATCC 87212) by replacement of the multiple cloning site with a ribosome binding site, intitiation codon and multiple cloning site from pTrc99C.
One of three vectors (ATCC 87214-87216) differing only in the reading frame of the multiple cloning site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862

Erhard Bremer, personal communication

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
吉木乃县| 东安县| 剑川县| 呼和浩特市| 富宁县| 屏山县| 祁连县| 民县| 肥东县| 太原市| 扎兰屯市| 江孜县| 土默特左旗| 蕉岭县| 丰顺县| 忻城县| 阳山县| 遵义市| 靖安县| 哈尔滨市| 青浦区| 前郭尔| 屏边| 农安县| 彝良县| 东乡县| 思南县| 金华市| 筠连县| 巴楚县| 新宁县| 万年县| 沧州市| 舞阳县| 木兰县| 泾源县| 清远市| 寿阳县| 济阳县| 哈密市| 鸡东县|