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pMMB278
pMMB278
規(guī)格:
貨期:
編號(hào):B236156
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pMMB278
商品貨號(hào) B236156
Designations pMMB278
Depositors VM Morales
Biosafety Level 1
Vector Information
Size (kb): 8.9899997711181640
Vector: pMMB278 (plasmid)
Promoters: Promoter tac
Construction: pMMB190, pMMB311
Marker(s):ampR
Construct size (kb): 8.989999771118164
Features: insert detection: lacZ'
marker(s): ampR
promoter: tac
replicon: oriV, oriT (IncQ)
repressor gene: lacIq
transcription terminator: rrnB
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.0; EcoRV--5.4, 3.7; SspI--5.0, 1.7, 1.5, 0.9; EcoRI/MluI--8.2, 0.8.
Contains sequences encoding lacIq, a tac promoter, and lacZalpha to allow direct screening by inactivation of alpha-complementation.
Constructed by replacing the MluI/EcoRI fragment of pMMB190 (taclacUV5 promoter) with the MluI/EcoRI fragment of pMMB311 (tac promoter).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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