国产成人综合久久久久久,亚洲国产成人久久综合碰,精品露脸国产偷人在视频,99久久精品免费看国产,国产免费一区二区三区在线观看,好吊妞国产欧美日韩免费观看,国产精品免费看久久久无码

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pHP45omega-Tc [JF297]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pHP45omega-Tc [JF297]
pHP45omega-Tc [JF297]
規(guī)格:
貨期:
編號:B244136
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pHP45omega-Tc [JF297]
商品貨號 B244136
Designations pHP45omega-Tc [JF297]
Depositors J Frey
Biosafety Level 1
Vector Information
Size (kb): 4.4800000190734860
Vector: pHP45omega-Tc (plasmid)
Construction: pHP45:omega, pBR322 tetR
Marker(s):ampR,tetR
Construct size (kb): 4.480000019073486
Features: marker(s): ampR, tetR
replicon: pMB1
terminator: T4D gene 32
Applications
integrating vector
shuttle vector
vector permitting positive selection for integration
vector useful for interposon mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.5, 2.4; HindIII--2.45, 2.3; SalI--4.9.
Because resistance levels may vary, it is important to determine the proper antibiotic concentration for each strain or isolate.
One of a series (ATCC 37653 through 37657) of vectors for in vitro mutagenesis (via the omega interposon) of gram-negative genera.
pHP45:omega was cut with BssHII + SphI (removing the spcR/strR gene) and the ends were made blunt. This was ligated to a blunt-ended 1.4 kb HindIII/AvaI fragment of pBR322 containing the tetracycline resistance gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Fellay R, et al. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52: 147-154, 1987. PubMed: 3038679

Frey J, Krisch HM. Omega mutagenesis in Gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species. Gene 36: 143-150, 1985. PubMed: 2998930

Shipped freeze-dried
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
黄大仙区| 剑阁县| 红桥区| 阳高县| 石楼县| 石阡县| 兴安县| 昆山市| 建平县| 平顺县| 塘沽区| 庐江县| 北票市| 平原县| 桦甸市| 巩留县| 衡东县| 彭山县| 酉阳| 明溪县| 团风县| 宿松县| 平阳县| 资阳市| 惠东县| 绥德县| 武夷山市| 汉中市| 乐陵市| 扎赉特旗| 循化| 龙里县| 安顺市| 民勤县| 宁晋县| 永昌县| 定州市| 盐源县| 岐山县| 平邑县| 江陵县|